The Mobile Medical Museum's permanent exhibit showcases over 300 years of local and world medical history, from the colonial period through the present. Collection highlights include pre-Civil War era anatomical models, early 20th century X-ray equipment, a functioning 1969 heart-lung machine, and an Emerson model iron lung from the 1930s.

The Robert Thrower Medicinal Garden highlights multicultural medicinal traditions that have survived around the world for thousands of years.  

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The Museum also organizes special exhibits on display in the Mary Elizabeth and Charles Bernard Rodning Gallery, and at various offsite locations in Mobile.